Posted on Jun 20, 2018, 11 p.m.
IBS affects about 1 in 6 people, an international team has provided clues to the organic triggers of irritable bowel syndrome.
Pathogenic factors of irritable bowel syndrome is an altered function of nerves in intestinal walls triggered by molecules released within the wall, this can be experimentally mimicked by mucosal biopsy supernatants from irritable bowel syndrome patients, whereas healthy control supernatants do not show nerve activation.
Supernatants were studied from patients with ulcerative colitis in remission for two reasons: Irritable bowel syndrome is considered as a mild form of inflammatory bowel disease; and patients with quiescence ulcerative colitis report irritable bowel syndrome like symptoms. Supernatants from these patients activate neurons.
Nerve activating properties of irritable bowel syndrome and ulcerative colitis supernatants were mainly due to proteases enzymes which are also signaling molecules. Nerve activation from irritable bowel syndrome supernatants was mediated by proteases signalling via protease activated receptor type 1, which played no role in effect of the ulcerative colitis supernatants.
An irritable bowel syndrome specific protein pattern, in particular an irritable bowel syndrome specific proteases profile has been found by the researchers. Proteome analysis revealed 204 differently expressed proteins in irritable bowel syndrome supernatants and 4 proteases which were only enhanced in irritable bowel syndrome supernatants. Protease inhibitor from probiotic Bifidobacterium longum strain blocked nerve activation triggered by irritable bowel syndrome supernatants. The interdisciplinary team over an 8 year period searched for putative biomarkers to identify organic changes in irritable bowel syndrome, and concluded that protease profiling holds promise as a strategy for identifying biomarkers of irritable bowel syndrome.
Materials provided by Technical University of Munich (TUM).
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Sabine Buhner, Hannes Hahne, Kerstin Hartwig, Qin Li, Sheila Vignali, Daniela Ostertag, Chen Meng, Gabriele Hörmannsperger, Breg Braak, Christian Pehl, Thomas Frieling, Giovanni Barbara, Roberto De Giorgio, Ihsan Ekin Demir, Güralp Onur Ceyhan, Florian Zeller, Guy Boeckxstaens, Dirk Haller, Bernhard Kuster, Michael Schemann. Protease signaling through protease activated receptor 1 mediate nerve activation by mucosal supernatants from irritable bowel syndrome but not from ulcerative colitis patients. PLOS ONE, 2018; 13 (3): e0193943 DOI: 10.1371/journal.pone.0193943